《植物生理学报》 2014, 50(12): 1769-1772

大麦游离小孢子的活力与耐盐性

刘成洪^1,2,*, 何婷^1,2,*, 王亦菲^1,2,**, 郭桂梅^1,2, 高润红^1,2, 徐红卫^1,2, 陈志伟^1,2, 杨沙沙^1,2,3, 黄亦辰^1,2,3, 陆瑞菊^1,2, 黄剑华^1,2

¹上海市农业科学院生物技术研究所, 上海201106; ²上海市农业遗传育种重点实验室, 上海201106; ³上海海洋大学水产与生命学院, 上海201306

通信作者：刘成洪；E-mail: cs1@saas.sh.cn；Tel: 021-62200524

摘　要：

取田间种植的大麦品种‘花30’幼穗游离小孢子, 采用荧光素二醋酸酯(FDA)染色法测定其在不同NaCl浓度胁迫下的活力, 用Image-Pro Plus软件对显微系统拍摄的照片进行分析计数, 研究了大麦离体小孢子对盐胁迫的耐受性。结果表明: 利用FDA染色结合软件显微拍照系统分析可以快速、准确地测定和分析游离小孢子的活力和大小。对本年度大田种植的大麦‘花30’材料, 按照成熟方法游离小孢子, 获得40%具有活力的小孢子, 游离小孢子在含1% NaCl提取液中至少2 h内活力保持稳定。

关键词：大麦; 游离小孢子; 细胞活力; 盐胁迫

收稿：2014-10-15   修定：2014-12-01

资助：大麦青稞产业技术体系(CARS-05)和上海市农业科学院学科建设专项(LY11)。 * 共同第一作者。

Viability and Salt Tolerance of Isolated Microspores from Barley (Hordeum vulgare)

LIU Cheng-Hong^1,2,*, HE Ting^1,2,*, WANG Yi-Fei^1,2,**, GUO Gui-Mei^1,2, GAO Run-Hong^1,2, XU Hong-Wei^1,2, CHEN Zhi-Wei^1,2, YANG Sha-Sha^1,2,3, HUANG Yi-Chen^1,2,3, LU Rui-Ju^1,2, HUANG Jian-Hua^1,2

¹Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; ²Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106, China; ³College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

Corresponding author: LIU Cheng-Hong; E-mail: cs1@saas.sh.cn; Tel: 021-62200524

Abstract:

The young spikes of barley (Hordeum vulgare) cultivar ‘Hua-30’ grown in field were used to isolate microspores, and cell viability of isolated microspores was measured under salt stress at different NaCl concentrations by the fluorescein diacetate (FDA) staining method. The salt tolerance of isolated microspores was evaluated based on the viability analysis by the software of Image-Pro Plus to capture the microscope pictures and count the cells. The results showed that it was convenient to analyze the microspore viability and size by cell staining with FDA and cell identification with the software. About 40% viable microspores could be achieved from barley cv. ‘Hua-30’ grown in field by established isolation procedures, and the isolated microspores were viable in the extraction buffer containing 1% NaCl for at least 2 h after isolation.

Key words: barley (Hordeum vulgare); isolated microspores; cell viability; salt stress